Credit Seminar on Elucidation of the role of rice sucrose transporter gene family in root exudation

Root exudates are the chemicals secreted into the soil by roots which regulate the soil microbial community in their immediate vicinity. Root exudates give resistance to pathogen, combat pathogenic micro-organism, and encourage beneficial symbioses by attracting beneficial micro-organism. The SUT genes if highly expressed may affect the sucrose exudation level in the roots. Comparison of the differences between the sucrose exudation profile of the wild type tobacco plant and that of overexpressed transgenic tobacco plants can be done to check this hypothesis. Investigation of the sucrose exudation profile in relation to SUT genes of rice can be done by overexpressing these genes in the tobacco plants. Further investigations can be done by gene knockout of tobacco plants’ SUT genes highly homologous to rice sucrose transporter genes and then extrapolated to rice sucrose transporter genes to elucidate the key functions of rice SUT genes. And finally the reverse transcription of all of the rice sucrose transporter genes and then check the sucrose exudation level via roots with the help of HPLC method. The use of fertilizers has resulted in loss of plants’ ability to acquire nutrients. SUT genes analyses/overexpression can help increase plant performance, resistance and nutrient activity. This can further help in elucidation of increased plant performance, altering role of root exudates, by using gene responsible for particular exudates in economically important crops.

Name:Mr. Sarbesh D. Dangol
M.Tech II Year Department of Genetic Engineering
Venue: Room number 701
Time:3:10 PM