Mycobacterium tuberculosis (MTB) is a pathogenic bacterial species that causes the infectious disease tuberculosis. Isoniazid and rifampicin are two major anti-tuberculosis drugs to which the organism has gained resistance and cause MDR-TB. This has raised the need for development of new metabolites or inhibitors that target the growth of MTB, which has an unusual waxy coating of primarily mycolic acid on the cell surface that contributes to the host-pathogen interaction and pathogenicity. The antagonist effect of the inhibitory agents produced by microorganismsEscherichia coli, Actinomycetes were studied. These test microorganisms were found to produce colicin, pyocyanin and thiolactomycinmetabolites respectively which were known to inhibit the growth of MTB. The normal growth characteristics of MTB and other three test organisms were read at 640nm using spectrophotometer. The inhibitory action of isolated metabolites against MTB was observed using spectrophotometer. Confirmation of the antimicrobial activity of the metabolites against MTBwas carried outby disc diffusion method on Middle brook 7H10 agar medium. Then the effect of these inhibitors against Mycobacterium tuberculosis was compared among which Colicin from E. coli and Thiolactomycin from Actinomycetes were found to be more effective.
Name: V.K.Prasanna M.Tech II Year
Reg .No: 1741110009
Guide Name: Mr.N.Manojkumar
Date: 24.09.12
Time: 9.15 AM
Venue: 701(B)