Zinc-finger Nuclease Mediated Plant Transformation

Gene targeting is a powerful tool for functional gene studies. Custom-designed zinc finger nucleases (ZFNs), proteins designed to cut at specific DNA sequences, are becoming powerful tools in gene targeting- the process of replacing a gene within a genome by homologous recombination (HR). These nucleases bind to the specific DNA recognition sites and introduce double stranded breaks (DSBs). Repair of these DSBs by normal cellular processes can be exploited to either disrupt genes or significantly increase the frequency of homologous recombination with a user-defined repair template. The creation of designer ZFNs that cleave DNA at a pre-determined site depends on the reliable creation of ZFPs that can specifically recognize the chosen target site within a genome. The development of ZFN-mediated gene targeting provides molecular biologists with the ability to site-specifically and permanently modify the plant and the mammalian genomes. Here, I discuss about the work done by various researchers on the potential of ZFN-mediated gene targeting with respect to animals as well as in plant species like in Soybean plant genes (Glycine max) and Arabidopsis plant. Also, it is mentioned about the applications of ZFNs and some of their limitations and risk associated.

Reg No: 1741210015
Batch: M.Tech II Year Genetic Engineering
Date: 23.07.13
Time: 9.00AM