The induction of double-strand breaks (DSBs) in plant genomes can lead to site-specific recombination at repair site.. This phenomenon has the potential for use in gene targeting applications in plant cells upon the induction of site-specific genomic DSBs using zinc finger nucleases (ZFNs). Zinc finger nucleases are artificial restriction enzymes, custom-designed to cleave a specific DNA sequence. Many desired custom-designed ZFNs are generated with high sequence-specificity and affinity. The development of ZFN-mediated gene targeting provides molecular biologists with the ability to site-specifically and permanently modify plant via homology-directed repair of a targeted genomic DSB.
Name of the Student:V.Bhavadharini
Reg No:1741210046
Batch:M.Tech II Year
Name of the Guide :Dr. M. Parani