Hands-on workshop on " Rolling Circle Amplification to clone circular viral genome"

 Rolling Circle Amplification to clone circular viral genome

Rolling circle amplification (RCA) is an isothermal enzymatic process in which a random

primer is used to amplify a circular DNA template by a special DNA polymerase called phi29

DNA polymerase. In recent years, this technique has been used for the efficient amplification

of circular DNA viral genomes without the need of specific primers. The viral genomes can

be further cloned for characterisation and infectious genomic clones can be generated directly

from the samples. This will help in the detection of novel viruses. During the workshop, the

participants will be learning the techniques involved in the process of cloning circular DNA

viral genome by rolling circle amplification. For more details, please check the brochure.

Interested students can register by using the following link